The vortex not only provides a different profile of mixing, but has the power to mix viscous fluids that would otherwise remain virtually unmixed (glycerol stocks come to mind). Vortex shakers are contra-indicated for some lab protocols (like in miniprep kits) …

Nov 29, 2016 · Never vortex the BSA solution. The best way to prepare a BSA solution is to put the BSA powder on the buffer solution (or water) instead of the other way round. Tap or rock the tube to dissolve the BSA. If there are some tiny clumps then pipette very gently (use 1ml tips; cut the tips if needed) but tapping/rocking should usually do the job.

Mar 4, 2013 · Assuming that you are talking about E. coli: As long as you are resuspending the cells in a suitable liquid, e.g. fresh medium or buffer, then from my experience you don't have much to worry about - the cells are very robust.

Jun 27, 2016 · Ugh. Did an immunofluorescence experiment last weekend, forgot to vortex both my primary and my secondary antibody solutions. And my final result looks dimmer than it should be. Is it possible that not vortexing contributed to this? I guess the question is, is vortexing really necessary? It would come down to the fluid dynamics.

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Vortex & lightly spin down on a centrifuge. Add the required amount of TAQ to master mix (insert in to the liquid) and mix using the pipette to draw some liquid up and down. Add master mixes to labeled PCR tubes in the required volumes as specified by the protocol.

Nov 27, 2019 · $\begingroup$ Your vision of an answer seems to me more of a nightmare. This is an example of a representation that would be totally inappropriate to the question you ask.

I'll add an informal answer to complement @Remi.b's excellent answer. In a very simple sense, you can think of the effective population size as the number of reproducing (breeding) individuals in a population.

Oct 23, 2013 · Stack Exchange Network. Stack Exchange network consists of 183 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

May 5, 2015 · Apologies if this question it too open-ended; evolutionary biology is not my primary field. I have been reading a lot about the use of statistical mechanics in analyzing evolutionary dynamics. As an