Jun 10, 2024 · Evolved and engineered Bxb1 recombinase variants (evoBxb1 and eeBxb1) mediated up to 60% donor integration (3.2-fold that of wild-type Bxb1) in human cell lines with …

The present disclosure provides evolved and engineered Bxbl recombinase variants. The recombinase variants provided herein exhibit increased recombination activity, for example, …

Mar 31, 2022 · We developed an efficient, precise, single-copy integration of large transgenes directly into zygotes using multiple mouse genetic backgrounds.

Mar 31, 2022 · We developed an efficient, precise, single-copy integration of large transgenes directly into zygotes using multiple mouse genetic backgrounds.

Oct 10, 2022 · In this study, we developed a computational approach to identify thousands of LSRs and their DNA attachment sites, expanding known LSR diversity by >100-fold and …

Jul 20, 2022 · Here, we develop an integrated experimental and computational method to engineer the DNA attachment sequence attP for predictably modulating the inversion reaction …

Oct 24, 2019 · Here we present a library of >20 orthogonal inducible split recombinases that can be activated by small molecules, light and temperature in mammalian cells and mice. …

Jan 1, 2017 · In this study, we compared the site-specific in vitro recombination systems of ϕRv1, ϕBT1, TG1, and Bxb1 based on purification of the above four integrases. The site-specific …

Nov 3, 2023 · While sufficient for many studies, the extent of genetic manipulation possible with a single recombinase site is limiting and insufficient for more complex cell-based assays. Here, …

Jan 18, 2019 · Here, we show that a point mutation (Bxb1-GA) in Bxb1 target sites significantly increases Bxb1-mediated integration efficiency at the Rosa26 locus in Chinese hamster ovary …