• Mel Blanc as Bugs Bunny, Daffy Duck, and Elephant
• Arthur Q. Bryan as Elmer Fudd (uncredited)

Rabbit Fire is generally considered among Chuck Jones and Michael Maltese's best works, and is noted for its use of dialogue gags in lieu of the physical gags more typical in animation. Besides the two sequels to this film, a number of other Jones shorts, including Beanstalk Bunny and Ali Baba Bunny, paired quick-witted Bugs and self-serving Daffy with (or rather against) each other.

It is also worth noting that in this episode, Mel Blanc showcased his ability to make one character imitate another character's voice, in this case, Daffy Duck impersonating Bugs Bunny and vice versa. Actor and voice actor Hank Azaria pointed out that as a voice actor, it is almost impossible to accomplish. At one point, he tried to do that in The Simpsons, along with the other cast members, but none of them could do it.

The "rabbit season/duck season" argument from this short became one of the references in the Looney Tunes franchise to have been analyzed both by scholars and by Jones himself (although this gag was actually used by Daffy against Porky six years earlier in the cartoon Duck Soup to Nuts). According to an essay by Darragh O'Donoghue, Rabbit Fire "stands in close relation to human experience, striving and generally failing to grasp an elusive quarry or goal." Richard Thompson said that in the film, there is "the clearest definition of character roles: Elmer never knows what's going on; Bugs always knows what's going on and is in control of things; Daffy is bright enough to understand how to be in control, but never quite makes it." Jones himself refers to Rabbit Fire as a "corner" picture, among his works that, "as in turning a corner in a strange city, reveal new and enchanting vistas."

The short earned an honorable mention for animation historian Jerry Beck's list of The Fifty Greatest Cartoons: As Selected by 1000 Animation Professionals. Its 1952 sequel, Rabbit Seasoning, made the actual list at number 30. The style, setup, and plot of Rabbit Fire were adapted into the opening sequence of Warner Bros.' 2003 film Looney Tunes: Back in Action.

The non sequitur elephant character based on Joe Besser was the inspiration for Horatio the Elephant, a recurring character on PBS' Sesame Street.

The Elephant from The Major Lied 'Til Dawn reappeared, but was redesigned.

• In two interviews conducted years after this cartoon was first released, director Chuck Jones fondly recalled voice artist Mel Blanc improvising hilariously as Daffy when he was trying to think of another word besides "despicable". However, in the finished film, only the words from the original dialogue script Archived 2012-02-07 at the Wayback Machine actually appear. Historians believe that Blanc did indeed improvise, as Jones remembered, but then Jones had decided instead to use what was originally written.
• Rabbit Fire and its two sequels often have two characters in the same frame for some length of time — an atypical aspect of the "Hunting" trilogy. In order to keep budgets under control, most Warner Bros. cartoons would cut back and forth between characters, rather than put two or more in the same shot. Or, at least, both characters might be in the same shot, but only one would actually be animated.
• Although the film is introduced by the Looney Tunes music The Merry-Go-Round Broke Down, the opening card indicates a Merrie Melodies "Blue Ribbon" release from 1960, and the end card is Merrie Melodies, replacing the original orange-red Looney Tunes title sequences.
• It marked the first cartoon where Bugs and Daffy starred and appeared together. While Bugs had made a cameo in Porky Pig's Feat (which co-starred Daffy and Porky Pig), this was the first where both were the stars.
• Although this is the first cartoon with Daffy's selfish side replacing his screwball side, he still hollers "hoo-hoo", a catchphrase from his screwball personality.